Reconfigurable Surfaces Employing Linear-Rotational and Bistable-Translational (LRBT) Joints

Reconfigurable surfaces are useful in many applications. This paper proposes a type of reconfigurable surfaces that consist of rigid elements (tiles) connected by novel compliant joints. Depending on the actuation, these novel connecting joints can either operate as torsional hinges, which create isometric transformation (like origami folding) between connected tiles, or bistable translational springs, which accommodate metric-changing transformation between connected tiles. A specific example of a reconfigurable surface with square tile shape that can morph into flat, cylindrical (in two different directions), and spherical configurations with simple actuation is given

Reconfigurable Surfaces Employing Linear-Rotational and Bistable-Translational (LRBT) Joints

Reconfigurable surfaces are useful in many applications. This paper proposes a type of reconfigurable surfaces that consist of rigid elements (tiles) connected by novel compliant joints. Depending on the actuation, these novel connecting joints can either operate as torsional hinges, which create isometric transformation (like origami folding) between connected tiles, or bistable translational springs, which accommodate metric-changing transformation between connected tiles. A specific example of a reconfigurable surface with square tile shape that can morph into flat, cylindrical (in two different directions), and spherical configurations with simple actuation is given

Liouville Perturbation Theory

A comparison is made between proposals for the exact three point function in Liouville quantum field theory and the nonperturbative weak coupling expansion developed long ago by Braaten, Curtright, Ghandour, and Thorn. Exact agreement to the order calculated (i.e. up to and including corrections of order O(g^{10})) is found.Comment: 6 pages, LaTe

First light of VLT/HiRISE: High-resolution spectroscopy of young giant exoplanets

A major endeavor of this decade is the direct characterization of young giant exoplanets at high spectral resolution to determine the composition of their atmosphere and infer their formation processes and evolution. Such a goal represents a major challenge owing to their small angular separation and luminosity contrast with respect to their parent stars. Instead of designing and implementing completely new facilities, it has been proposed to leverage the capabilities of existing instruments that offer either high contrast imaging or high dispersion spectroscopy, by coupling them using optical fibers. In this work we present the implementation and first on-sky results of the HiRISE instrument at the very large telescope (VLT), which combines the exoplanet imager SPHERE with the recently upgraded high resolution spectrograph CRIRES using single-mode fibers. The goal of HiRISE is to enable the characterization of known companions in the $H$ band, at a spectral resolution of the order of $R = \lambda/\Delta\lambda = 100\,000$, in a few hours of observing time. We present the main design choices and the technical implementation of the system, which is constituted of three major parts: the fiber injection module inside of SPHERE, the fiber bundle around the telescope, and the fiber extraction module at the entrance of CRIRES. We also detail the specific calibrations required for HiRISE and the operations of the instrument for science observations. Finally, we detail the performance of the system in terms of astrometry, temporal stability, optical aberrations, and transmission, for which we report a peak value of $\sim$3.9% based on sky measurements in median observing conditions. Finally, we report on the first astrophysical detection of HiRISE to illustrate its potential.Comment: 17 pages, 15 figures, 3 tables. Submitted to A&A on 19 September 202

Phylogeographic pattern and extensive mitochondrial DNA divergence disclose a species complex within the Chagas disease vector Triatoma dimidiata.

ABSTARCT: Previous studies have shown that "bioequivalent" generic products of vancomycin are less effective in vivo against Staphylococcus aureus than the innovator compound. Considering that suboptimal bactericidal effect has been associated with emergence of resistance, we aimed to assess in vivo the impact of exposure to innovator and generic products of vancomycin on S. aureus susceptibility. A clinical methicillin-resistant S. aureus (MRSA) strain from a liver transplant patient with persistent bacteremia was used for which MIC, minimum bactericidal concentration (MBC), and autolytic properties were determined. Susceptibility was also assessed by determining a population analysis profile (PAP) with vancomycin concentrations from 0 to 5 mg/liter. ICR neutropenic mice were inoculated in each thigh with ∼7.0 log(10) CFU. Treatment with the different vancomycin products (innovator and three generics; 1,200 mg/kg of body weight/day every 3 h) started 2 h later while the control group received sterile saline. After 24 h, mice were euthanized, and the thigh homogenates were plated. Recovered colonies were reinoculated to new groups of animals, and the exposure-recovery process was repeated until 12 cycles were completed. The evolution of resistance was assessed by PAP after cycles 5, 10, 11, and 12. The initial isolate displayed reduced autolysis and higher resistance frequencies than S. aureus ATCC 29213 but without vancomycin-intermediate S. aureus (VISA) subpopulations. After 12 cycles, innovator vancomycin had significantly reduced resistant subpopulations at 1, 2, and 3 mg/liter, while the generic products had enriched them progressively by orders of magnitude. The great capacity of generic vancomycin to select for less susceptible organisms raises concerns about the role of therapeutic inequivalence of any antimicrobial on the epidemiology of resistance worldwide

Direct characterization of young giant exoplanets at high spectral resolution by coupling SPHERE and CRIRES+

This is the final version. Available on open access from EDP Sciences via the DOI in this recordStudies of atmospheres of directly imaged extrasolar planets with high-resolution spectrographs have shown that their characterization is predominantly limited by noise on the stellar halo at the location of the studied exoplanet. An instrumental combination of highcontrast imaging and high spectral resolution that suppresses this noise and resolves the spectral lines can therefore yield higher quality spectra. We study the performance of the proposed HiRISE fiber coupling between the direct imager SPHERE and the spectrograph CRIRES+ at the Very Large Telescope for spectral characterization of directly imaged planets. Using end-to-end simulations of HiRISE we determine the signal-to-noise ratio (S/N) of the detection of molecular species for known extrasolar planets in H and K bands, and compare them to CRIRES+. We investigate the ultimate detection limits of HiRISE as a function of stellar magnitude, and we quantify the impact of different coronagraphs and of the system transmission. We find that HiRISE largely outperforms CRIRES+ for companions around bright hosts like β Pictoris or 51 Eridani. For an H = 3.5 host, we observe a gain of a factor of up to 36 in observing time with HiRISE to reach the same S/N on a companion at 200 mas. More generally, HiRISE provides better performance than CRIRES+ in two-hour integration times between 50–400 mas for hosts with H < 8.5 and between 50–800 mas for H < 7. For fainter hosts like PDS 70 and HIP 65426, no significant improvements are observed. We find that using no coronagraph yields the best S/N when characterizing known exoplanets due to higher transmission and fiber-based starlight suppression. We demonstrate that the overall transmission of the system is in fact the main driver of performance. Finally, we show that HiRISE outperforms the best detection limits of SPHERE for bright stars, opening major possibilities for the characterization of future planetary companions detected by other techniquesEuropean Union Horizon 202

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Initial Public Offerings and the Firm Location

The firm geographic location matters in IPOs because investors have a strong preference for newly issued local stocks and provide abnormal demand in local offerings. Using equity holdings data for more than 53,000 households, we show the probability to participate to the stock market and the proportion of the equity wealth is abnormally increasing with the volume of the IPOs inside the investor region. Upon nearly the universe of the 167,515 going public and private domestic manufacturing firms, we provide consistent evidence that the isolated private firms have higher probability to go public, larger IPO underpricing cross-sectional average and volatility, and less pronounced long-run under-performance. Similar but opposite evidence holds for the local concentration of the investor wealth. These effects are economically relevant and robust to local delistings, IPO market timing, agglomeration economies, firm location endogeneity, self-selection bias, and information asymmetries, among others. Findings suggest IPO waves have a strong geographic component, highlight that underwriters significantly under-estimate the local demand component thus leaving unexpected money on the table, and support state-contingent but constant investor propensity for risk